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anti obr antibody  (R&D Systems)


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    R&D Systems anti obr antibody
    Anti Obr Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+obr+antibody/Human+Leptin+R+Antibody/us11965028-257-19-22
    Average 93 stars, based on 5 article reviews
    anti obr antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Immunoprecipitation:

    Article Title: Neuropilin-1-peptide based antibody, highlighting new epitope associated to glycosaminoglycan-modified neuropilin-1 and methods of use thereof
    Article Snippet: Immunoprecipitation of NRP-1 in protein lysates 100 μg of protein lysate from the MDA-MB-231, COLO 205, CTC41.4 (circulating tumor cells isolated from patient suffering metastatic colon cancer—Origin of the cells: Laboratory of Rare Human Circulating Cells (LCCRH), Saint Eloi Hospital, University Medical Centre, Montpellier France)) and wild-type and NRP-1-K0 A549 (human lung cancer cell line) cells were incubated overnight at 4° C., with a mix (1:1) of protein A and protein G Sepharose 4 Fast Flow beads (17-6002-35, GE Healthcare) and 2 μg of different antibodies of the invention a-NRP1_LEP (mouse, chimeric and humanized variants), as well as prior art antibodies, a-NRP1_VE and a-NRP1_SEM, or their respective isotype controls. .. To investigate the NRP-1/0BR interaction in CTC41.4 cells, 100 μg of protein lysate was immunoprecipitated as described above with anti-OBR antibody (MAB867, R&D Systems). .. On the day after, the immunoprecipitated samples were washed three times with PBS, resuspended in 10% Beta-mercaptoethanol 4X Laemmli Sample Buffer (1610747, Bio-Rad), heated for 5 min at 95° C. and then stored at −20° C. or subjected to Western-blot analysis.



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    IL-6 and <t>LEPR</t> immunostaining in control and preeclamptic placentas. Representative images of IL-6 and LEPR immunostaining in preeclamptic (PE) and gestational aged-matched control placentas. Hofbauer cells-specific HSCORE analysis demonstrated significantly higher IL-6 (n = 5/group) and LEPR expression in PE placentas (n = 8) vs. gestational aged-matched controls (n = 7) (83.0 ± 20.9 vs. 25.0 ± 4.5 and 63.5 ± 18.6 vs. 10.9 ± 1.0, respectively). Arrows indicate IL-6 and LEPR-expressing Hofbauer cells. Bars represent median values, * p < 0.05 vs. control (C) specimens analyzed by Mann–Whitney Rank Sum test. Scale bar = 30 µm.
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    Image Search Results


    Pearson’s correlation (R) between anthropometric and biochemical variables and the mRNA expression (a.u.) of genes in study tissues.

    Journal: International Journal of Molecular Sciences

    Article Title: Evidence That Peripheral Leptin Resistance in Omental Adipose Tissue and Liver Correlates with MASLD in Humans

    doi: 10.3390/ijms25126420

    Figure Lengend Snippet: Pearson’s correlation (R) between anthropometric and biochemical variables and the mRNA expression (a.u.) of genes in study tissues.

    Article Snippet: The tissue was incubated overnight with a 1:10 dilution of anti-ObR/ LEPR antibody (B:3) (sc-8391; Santa Cruz Biotechnology, Dallas, TX, USA), followed by anti-mouse peroxidase-labeled secondary antibody.

    Techniques: Expressing, Fat

    Pearson’s correlation (R) between the mRNA expression (a.u.) of genes in study tissues.

    Journal: International Journal of Molecular Sciences

    Article Title: Evidence That Peripheral Leptin Resistance in Omental Adipose Tissue and Liver Correlates with MASLD in Humans

    doi: 10.3390/ijms25126420

    Figure Lengend Snippet: Pearson’s correlation (R) between the mRNA expression (a.u.) of genes in study tissues.

    Article Snippet: The tissue was incubated overnight with a 1:10 dilution of anti-ObR/ LEPR antibody (B:3) (sc-8391; Santa Cruz Biotechnology, Dallas, TX, USA), followed by anti-mouse peroxidase-labeled secondary antibody.

    Techniques: Expressing

    IL-6 and LEPR immunostaining in control and preeclamptic placentas. Representative images of IL-6 and LEPR immunostaining in preeclamptic (PE) and gestational aged-matched control placentas. Hofbauer cells-specific HSCORE analysis demonstrated significantly higher IL-6 (n = 5/group) and LEPR expression in PE placentas (n = 8) vs. gestational aged-matched controls (n = 7) (83.0 ± 20.9 vs. 25.0 ± 4.5 and 63.5 ± 18.6 vs. 10.9 ± 1.0, respectively). Arrows indicate IL-6 and LEPR-expressing Hofbauer cells. Bars represent median values, * p < 0.05 vs. control (C) specimens analyzed by Mann–Whitney Rank Sum test. Scale bar = 30 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis

    doi: 10.3390/ijms25010135

    Figure Lengend Snippet: IL-6 and LEPR immunostaining in control and preeclamptic placentas. Representative images of IL-6 and LEPR immunostaining in preeclamptic (PE) and gestational aged-matched control placentas. Hofbauer cells-specific HSCORE analysis demonstrated significantly higher IL-6 (n = 5/group) and LEPR expression in PE placentas (n = 8) vs. gestational aged-matched controls (n = 7) (83.0 ± 20.9 vs. 25.0 ± 4.5 and 63.5 ± 18.6 vs. 10.9 ± 1.0, respectively). Arrows indicate IL-6 and LEPR-expressing Hofbauer cells. Bars represent median values, * p < 0.05 vs. control (C) specimens analyzed by Mann–Whitney Rank Sum test. Scale bar = 30 µm.

    Article Snippet: LepR (ObR) , Santa Cruz #sc-8391(B-3) , IHC 1:100.

    Techniques: Immunostaining, Control, Expressing, MANN-WHITNEY

    Antibodies used in this study. WB: Western blotting, IHC: Immunohistochemistry.

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis

    doi: 10.3390/ijms25010135

    Figure Lengend Snippet: Antibodies used in this study. WB: Western blotting, IHC: Immunohistochemistry.

    Article Snippet: LepR (ObR) , Santa Cruz #sc-8391(B-3) , IHC 1:100.

    Techniques: Western Blot, Immunohistochemistry